A preliminary study on in vitro seed germination and rooted callus formation of Tetrastigma leucostaphylum (Family : Vitaceae) a host plant for Rafflesia

Surya, Netty W and Idris, M (2010) A preliminary study on in vitro seed germination and rooted callus formation of Tetrastigma leucostaphylum (Family : Vitaceae) a host plant for Rafflesia. In: 8th Flora Malesiana Symposium, Singapore, August 23-27, 2010, Singapore.

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Abstract

Abstract Propagation in vitro of Tetrastigma leucostaphylum as a host plant for Rafflesia is one of the alternatives that must be explored in a sustainable conservation effort for Rafflesia. This research was conducted as a preliminary study on propagation of T. Leucostaphylum. Two step of research was in vitro seed germination and inducing rooted callus formation. The result showed that T. leucostaphylum seed germination is epigeal with seedling emergence period ranging 30-60 days after planting (dap), regardless of presence or absence of light. Percentage of seed germinated after 60 days was 54-60% of the total seed that germinated in media treatments. Murashige-Skoog (MS) medium + 0,5 mg/L Kinetin was a better medium to promote seed germination. Callus formation began 7 dap on MS medium + 2 mg/L NAA. While, MS medium + 2 mg/L 2,4-D, callus formation began 21 dap. In this medium, a cutting part of hypocotyl resulted some phenolic that make the medium browning. Rooted callus formation was seen after 21 days on MS medium + 2 mg/L NAA and it was not evident with the addition of 2 mg/L 2,4-D. Callus producing roots is a better source for germination and infection of Rafflesia seed for the next research. Key words : in vitro germination, Tetrastigma leucostaphylum, rooted callus

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