Harpahmi, Harpahmi
(2014)
PENGARUH LEVEL GLISEROL DAN WAKTU EQUILIBRASI YANG
BERBEDA TERHADAP KUALITAS SPERMATOZOA KERBAU.
Masters thesis, Universitas Andalas.
Abstract
Four ejaculates obtained from a buffalo (age 3 year old) in BIBD Tuah Sakato Paykumbuh were used to study effect of different concentration of glycerol and different time of equilibration on post thawing sperm motility, percentage of living sperm, percentage of abnormality and plasma membrane integrity. Semen was diluted with tris citrate eeg yolk and five different concentration of glycerol. The experiment used a Completely Randomized Block Design with two factor, factor A is different concentration of glycerol (6%, 7%, 8%, 9% and 10%), factor B is different time of equilibration (3, 5 and 7 hours). The results showed that post thawing sperm motility, percentage of living sperm, percentage of abnormality and plasma membrane integrity for factor A (different concentration of glycerol) were highly significant (P<0.01). The effect of factor B (different time of equilibration) showed that there were higly significant (P<0.01) on post thawing sperm motility, percentage of living sperm, and plasma membrane integrity but showed were significant (P<0.05) on post thawing abnormality. In conclusion that the level glycerol at 7% concentration and time of equilibration at 5 hours were better in protecting the quality of the spermatozoa than other treatments.
Keywords : Glycerol, equilibration, sperm quality.
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