Krisyaneli, Krisyaneli
(2014)
KARAKTERISASI SIMPLISIA DAN EKSTRAK SERTA ISOLASI
SENYAWA AKTIF ANTIBAKTERI DARI DAUN KARAMUNTING.
KARAKTERISASI SIMPLISIA DAN EKSTRAK SERTA ISOLASI SENYAWA AKTIF ANTIBAKTERI DARI DAUN KARAMUNTING ( Rhodomyrtus tomentosa ( W.Ait ) Hassk )
.
(Submitted)
Abstract
Abstract
Karamunting has traditionally been used to cure some infection diseases, such as diarrhea.
The objective of this research is characterized the simplisia of leaves karamunting, and find it’s
antibacterial compound that can be used as marker compound. An antimicrobial compound was
isolated from ethyl acetate extract from characterized dried leaves of Karamunting (Rhodomyrtus
tomentosa (W.Aitt) Hassk). From IR, UV and NMR spectrum known that the isolate is
Rhodomyrtosone C, an acylphloroglucinol. It was obtained as yellowish solid form, with
molecular formula C41H54O8. This isolate has antimicrobial activity towards Salmonella
thypimurium ATCC 14028 and Eschericia coli ATCC 25992, but not toward Vibro
parahaemolyticus 8070. The antimicrobial activity and Minimum Inhibitory Concentration
measurement are determine with dilution method using 96 well microtitter plate. The MIC of
isolate against E.coli, S.thypimurium and V.parahaemolyticus were 3.125; 6.25 and 6.25 ppm
respectively. The result of the equivalence test of isolate using Ciprofloksazin showed that 1μg of
isolate was equivalent to 0.018 ; 0.14 and 4.11 x 10-5 μg/mL of Ciprofloksazin towards those
bacterias respectively. The isolate concentration measurement toward extract and simplisia done
by using HPLC Shim-pack VP ODS (250 x 4.6 mm), normal phase, using methanol 100% as eluen
with flow rate 1 mL/minute, and the result showed that the isolate rate from ethyl acetate extract
was 8.488 % ± 0.27 and 0.2771 % ± 0.008 toward characterized simplisia.
Keyword: R.tomentosa, antibacterial, MIC, rhodomyrtosone C
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