PRODUKSI BIOETANOL DARI AMPAS SAGU (Metroxylon sp) MELALUI PROSES PRETREATMENT DAN METODE SIMULTANEOUS SACCHARIFICATION FERMENTATION (SSF)

Khairunnisah, Khairunnisah (2014) PRODUKSI BIOETANOL DARI AMPAS SAGU (Metroxylon sp) MELALUI PROSES PRETREATMENT DAN METODE SIMULTANEOUS SACCHARIFICATION FERMENTATION (SSF). PRODUKSI BIOETANOL DARI AMPAS SAGU (Metroxylon sp) MELALUI PROSES PRETREATMENT DAN METODE SIMULTANEOUS SACCHARIFICATION FERMENTATION (SSF) . (Unpublished)

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Abstract

Abstract Sago waste is one of biomass lignocellulose that potential to produce bioethanol. Lignocellulose material consist of three major component such as cellulose, hemicellulose and lignin. The available of lignin become inhibitor for enzymatic process it must removed by pretreatment. Research for bioethanol production from sago waste through pretreatment process by using a variation of alkaline solution; 1% NaOH, 8% NH4OH, 1% NaOH + 4% NH4OH and 1% NaOH + 8% NH4OH with a ratio solid to liquid 1:10 (w/v) after that, followed by SSF method involved two kind of fungi like Trichoderma viride strain T1 sk for saccharification and Saccharomyces cerevisiae for fermentation process. The optimum conditions for the reduction of samples obtained after pretreatment at concentration of 1% NaOH + 8% NH4OH at 49,3017% with a long incubation period of 3 days with a temperature 500C. Result of determination cellulase enzyme activity from Trichoderma viride strain T1 sk by 0.1% CMC is 0,1144 unit and the highest glucose yield is 921,25 μg/mL from 0,9 gram substrate of sago waste while the optimum time for the saccharification process obtained in 75 minutes. Ethanol content was analyzed by GC/MS resulted 12.99% (% area) or equal with 0,38 mL ethanol yield for 168 hours of fermentation . Keywords : Sago waste, bioethanol, alkaline pretreatment, SSF

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