Putri, Melysa (2014) PENGARUH PENAMBAHAN NaOH-NH4OH UNTUK PRODUKSI BIOETANOL DARI AMPAS TEBU DENGAN METODE SIMULTANEOUS SACCHARIFICATION FERMENTATION (SSF). PENGARUH PENAMBAHAN NaOH-NH4OH UNTUK PRODUKSI BIOETANOL DARI AMPAS TEBU DENGAN METODE SIMULTANEOUS SACCHARIFICATION FERMENTATION (SSF) . (Unpublished)
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Abstract
Bagasse can be used as a basic ingredient in the manufacture of bio-ethanol and cellulose in bagasse first should be released from lignin by using pretreatment method. Pretreatment was done by using a mixture of 2% NaOH with NH4OH concentrations that varied from 2% to 10%. The solids ratio variation (bagasse): liquid (NaOH-NH4OH) pretreatment was 1:10, 1:15, and 1:20. Pretreatment condition which provided the optimum sample weight reduction was at concentration of 2% NaOH and 2% NH4OH with a ratio of 1:20. The results of bagasse saccharification using cellulase enzyme crude extract of Trichoderma viride strain T1 sk against CMC pure substrates showed enzyme activity of 0,188 units. Saccharification was also done to the variation of the amount of bagasse substrate 0,1g to 1 g and the variations of the time of saccharification from 30 min to 120 min. The maximum glucose concentration was 1528.57 µg / mL which obtained at 0.6 g substrate of bagasse with a long of saccharification 60 minutes. Bio-ethanol obtained by the method of SSF for 84 hours with 30 mL inoculum of Saccharomyces cerevisiae and 10 mL of crude extract of the enzyme cellulase was determined by using gas chromatography, is equal to 0,4278 mL. Keywords: bagasse, pretreatment, SSF
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QD Chemistry |
| Unit atau Lembaga: | Fakultas MIPA > Kimia Paca Sarjana > Doktor > Fakultas MIPA > Kimia Fakultas MIPA > Kimia |
| Depositing User: | Riani Tri Utami |
| Date Deposited: | 02 Jun 2014 00:16 |
| Last Modified: | 02 Jun 2014 00:16 |
| URI: | http://repository.unand.ac.id/id/eprint/20475 |
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