Perbanyakan tanaman gaharu (Aquilaria malaccensis L ) sebagai upaya pelestarian plasma nutfah secara in vitro (Plant Propagation Agarwood (Aquilaria malaccensi L ) by Effort Conservation Plasma Nutfah to in Vitro)

Frizia, Fevi and Yusniwati, Yusniwati and Muhsanati, Muhsanati (2014) Perbanyakan tanaman gaharu (Aquilaria malaccensis L ) sebagai upaya pelestarian plasma nutfah secara in vitro (Plant Propagation Agarwood (Aquilaria malaccensi L ) by Effort Conservation Plasma Nutfah to in Vitro). Working Paper. Fakultas Pertanian. (Unpublished)

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Abstract

Experiment to invistigate (Plant Propagation Agarwood ((Aquilaria malaccensi L ) by Effort Conservation Plasma Nutfah to in Vitro) was done in February to September 2006 at tissue cultur laboratory of Agriculture Faculty, University Andalas Padang. The experiment consist of two serries, The first serries about give concentration 2,4-D for induction callus, seven (7) level consist of : 0,00 ppm (A1); 5,00 ppm (A1); 10,00 ppm (A2); 15,00 ppm (A3); 20,00 ppm (A4); 25,00 ppm (A5); dan 30,00 ppm (A6) The second serries about give combination concentration NAA and BAP for regeneration callus,seven (7) level consist of : 0,00 ppm NAA + BAP + 0,10 Kinetin (K0); 0,50 ppm NAA + 0,10 ppm Kinetin + 1,75 ppm BAP(K1); 0,50 ppm NAA + 0,10 ppm Kinetin + 3,50 ppm BAP(K2); 0,50 ppm NAA + 0,10 ppm Kinetin + 5,25 ppm BAP(K3); 1,0 ppm NAA + 0,10 ppm Kinetin + 1,75 ppm BAP(K4); 1,0 ppm NAA + 0,10 ppm Kinetin + 3,50 ppm BAP(K5); dan 1,0 ppm NAA + 0,10 ppm Kinetin + 5,25 ppm BAP(K6) The experiment used Completely Ramdomized Design (CRD) with four (replication). Concentration 15,00 ppm 2,4-D (A3) were supperior result than any another treatment the support growth to get explant life, callus formation , and less browning. Combination concentration 1,00 ppm NAA + 3,50 ppm BAP (K5) were supperior result than any another treatment the support growth to get callus life, and regeneration callus formed shootlet.

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