Development and validation of thin layer chromatography-densitometry method for analysis of mefenamic acid in tablet

Rivai , Harrizul and Putri, Wery Kurnia and Armin, Fithriani (2016) Development and validation of thin layer chromatography-densitometry method for analysis of mefenamic acid in tablet. Journal of Chemical and Pharmaceutical Research, 8 (1). pp. 565-570. ISSN 0975-7384

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Abstract

Mefenamic acid is routinely used as tablet dosage forms. Thin layer chromatography (TLC) promotes for higher separation efficiencies, shorter analysis time, lower amounts of mobile phase, and efficient data acquisition and processing. There are various analytical methods for their estimation of mefenamic acid but till date there is no TLC method for its analysis. The paper presents the development and validation of a new TLC method for analysis of mefenamic acid in tablet. Separation was performed on silica gel 60 F254 plates. The mobile phase is comprised of chloroform: methanol (9.0: 0.1, v: v). Densitometry evaluation of the separated zones was performed at 320 nm. The drug was satisfactorily resolved with RF values of 0.55 ± 0.03. The accuracy and reliability of the me thod was assessed by evaluation of linearity (50-300 µg/mL), precision intra-day and inter-day RSD values were always less than 2, accuracy (102.45 % ± 1.36% for Sample A and 100.28 % ± 1.90 % for Sample B) in accordance with ICH guidelines. The proposed method is new, accurate and precise. Therefore, it is suitable for determination of mefenamic acid in tablet for analytical and pharmaceutical purposes.

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