STRONG ANTIMICROBIAL OF LACTIC ACID BACTERIA AND SPECIES IDENTIFICATION OF VIRGIN COCONUT OIL PRODUCTS IN PADANG WEST SUMATERA, INDONESIA

Rajagukguk, Horas and Syukur, Sumaryati and Syafrizayanti, Syafrizayanti and Syaputri, Yolani and Purwati, Endang and Iwahashi, Hitoshi (2017) STRONG ANTIMICROBIAL OF LACTIC ACID BACTERIA AND SPECIES IDENTIFICATION OF VIRGIN COCONUT OIL PRODUCTS IN PADANG WEST SUMATERA, INDONESIA. In: Greetings from 4 th International Conference on Earth Sciences and Engineering (4 International Conference on Earth Sciences and Engineering (4 th ICEE 2017) . (Submitted)

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Abstract

Virgin Coconut Oil (VCO) products are available in the market of Padang west Sumatra Indonesia, Produced by lokal Home Industry. Coconut, Cocos nucifera, is an important member of the family Arecaceae, have been traditional used for food and medicine in peoples of West Sumatra. Differently with coconut itself, the oil of coconut , VCO not all accepted to use as food supplement because of the image of the oil will increase blood cholesterol. Recently several reports proved that VCO contain Medium Chain Fatty Acids, as Lauric Acids is metabolize quickly to produce high energy and has antimicrobial activities. Lactic Acids Bacteria, known as antimicrobial also, have been isolated from 7 VCO samples, and 4 samples have strong lactic acid bacteria as antimicrobial. All of isolates VCO in MRS medium are gram positive, catalase negative and homofermentative. All isolates are able to inhibit pathogen bacteria such as Escherichia coli, Bacillus subtilis and Staphylococcus aureus. Antimicrobial test was obtained, the largest inhibition zones from pathogen bacteria are by sample codes 1A (12 mm), 6C (13.5 mm), and 3A (15 mm), respectively. Ampicillin Sodium 100 μg/mL is used as a positive control. All isolates can inhibit pathogenic bacteria in pH range of 3-9, but gave negative results on pH 3 against S. aureus. All of Lactic Acid Bacteria was obtained to sequence analysis using 16 S rRNA showed that Lactobacillus plantarum strain PON100536, Lactobacillus plantarum strain C410L1, Lactobacillus plantarum strain MF1298, Lactobacillus plantarum strain LY-78, Lactobacillus plantarum strain NM22-22, Lactobacillus plantarum strain DS2 KCTC12992BP, and Lactobacillus plantarum strain L41 with concentration Lactic acid 0.2725, 0.2775, 0.2950, 0.3050, 0.31, 0.2925, 0.3050 M, respectively.

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