IDENTIFIKASI KULTIVAR-KULTIVAR KENTANG MELALUI ANALISIS ISOENZIM (Identification of Potato Cultivars by Isoenzyme Analysis)

Suliansyah , Irfan (2010) IDENTIFIKASI KULTIVAR-KULTIVAR KENTANG MELALUI ANALISIS ISOENZIM (Identification of Potato Cultivars by Isoenzyme Analysis). Jurnal Stigma, XIII (3). ISSN 0853-3776

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Abstract

In plant breeding programs, information on genetic diversity within and among closely relate crop species is essential for particularly in characterizing individual accessions and cultivars. Genetic characterization can be morphological marker, protein marker (isoenzyme), as well as molecular markers. Genetic study within and among species with protein marker (isoenzyme) more accurately than morphological marker, because morpho-logical characters usually vary with environments. The protein (isoenzyme) polymorphism detected by electro-phoresis way, and difference between alleles revealed from charge amino acids replacement. 15 cultivar of potato: Prevalent, Granola, Red Pontiac, Carni 1, Carni 2, Alpha, BF 15, PAS 4062, Kennebec, S. chacoense, Astarte, Noorchip, PAS 3063, Atlantic, dan Atzimba were objected in this study. Method of isoenzyme analysis base on Wendel dan Weeden (1989) tehnique. Each accession product was considered to be a unit character, which the accession were scored for the presence (1) or absence (0) of a product. The similarity indices were converted into dissimilarity. The resulting dissimilarity matrix was subjected to hierarchical cluster analysis using the Unweighted Pair Group Method and Arithmetic Average (UPGMA) and was done using computer program NTSYS-pc, version 1.80 (Exeter Software, New York). The result demonstrated that isoenzyme analysis is able to reveal genetic difference between potato cultivar. The gene controlling isoenzyme of peroxide dan esterase has two locus. Based on similarity analysis the potato cultivar consist of a genotype group and free individual/genotype. Key words: potato, cultivars, isoenzyme analysis

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