ANALISIS ISOENZIM SEBAGAI PENANDA BIOKIMIA UNTUK MENGKARAKTERISASI TANAMAN GAMBIR (Uncaria gambir (Hunter Roxb))

Syukriani, Lily and Kusumawati , Aries (2008) ANALISIS ISOENZIM SEBAGAI PENANDA BIOKIMIA UNTUK MENGKARAKTERISASI TANAMAN GAMBIR (Uncaria gambir (Hunter Roxb)). Jerami, 1 (2). ISSN 1979-0228

Microsoft Word (ANALISIS ISOENZIM SEBAGAI PENANDA BIOKIMIA UNTUK MENGKARAKTERISASI TANAMAN GAMBIR (Uncaria gambir (Hunter Roxb))) - Published Version Available under License Creative Commons Public Domain Dedication. Download (409Kb)

Abstract

An experiment was aimed at determining suitable isoenzymes as genetic marker in characterising three genotypes of gambier plants, i.e. Udang, Cubadak, and Riau. These genotypes were compared to other six genotypes of gambier of the collection of Faculty of Agriculture, Andalas University. Gambier leaves were subjected to analytical method following a modified-technique of Wendel dan Weeden (1989). Isoenzymes analysis was carried out as the following stages: preparation of buffer, preparation of starch gel, enzyme extraction, electrophoresis, and staining. Results demonstrate: 1) isoenzyme estherase, proxidase, and ACP can be used to determine genetic relatedness and grouping in gambier plants; 2) gene controlling isoenzyme estherase, proxidase, and ACP were identified at two loci, and 3) there were three groups of gambier genotype found according to the relatedness analysis of isoenzyme estherase at 36.75% similarities; isoenzyme peroxidase at 29.29% similarities shown two groups of gambier genotypes; and isoenzyme ACP at 36.75% similarities revealed three groups of gambier genotypes. Key words:Isoenzyme, characterization, gambir

Actions (login required)

View Item