REGENERASI KALUS MANGGIS (Garcinia mangostana L.) MELALUI KULTUR IN VITRO(Regeneration Callas Mangosteer (Garcinia mangostana Z.) By In Vitro Culture)

Satria, Benni and Dwipa , Indra and Jamsari, Jamsari (2010) REGENERASI KALUS MANGGIS (Garcinia mangostana L.) MELALUI KULTUR IN VITRO(Regeneration Callas Mangosteer (Garcinia mangostana Z.) By In Vitro Culture). Stigma, VII (1). ISSN 0853-3776

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Abstract

Experiment regeneration callus mangosteen(Garcinia mangostana L.) by in vitro culture was done in June 1998 to January 1999 at Tissue Culture Laboratory of Agriculture Faculty, University of Andalas Padang. This experiment studies about combination conoentration Auxin and cytokinin plant $owth regulator that exactly support growth regeneration callus of mangosteen explant. to get plantlet by in vitro. The experiment used completely Randomized Design (CRD) with 5 replication. Stage regeneration callus on Woody Plant Medium (WPM) with combination concentration Auxin and Cytokinin plant growth regulator 5 level consist of:(Z1 0.50 ppm NAA + 1.75 ppm BAP.(%) 0.50 ppm NAA+3.00ppmBAP.(Z3 0.25ppmNAA +0.10ppmKinetin+ 1.75ppmBAP.(Z4)0.50ppmNAA + 3.00 ppm BAP . (k) 1.00 ppm 2.4-D + 0.10 ppm NAA + 25 % water coconut,Combination concentration 0.50 ppm NAA + 0.10 ppm Cynetin + 3.00 ppm BAP (Z4) were supperior result than any another ffeatment the support growth regeneration callus. Percentage initiation callus, shootlet formation, rootlet formation, plantlet formation and explant life were higher on combination concentration 0,50 ppm NAA + 0,10 ppm Cynetin + 3,00 ppm BAP (Za).

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