AMOBILISASI ENZIM ENDOGLUKANASE (Cx)DARI Volvariella valvacea

Agustien, Anthoni (2010) AMOBILISASI ENZIM ENDOGLUKANASE (Cx)DARI Volvariella valvacea. JUMPA JURNAL MATEMATIKA DAN PENGETAHUAN ALAM, 4 (1). ISSN 0853-0033

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Abstract

Cellulase is very interesting multienzyme because ithydrolyzed cellulose substrate,the main component of agriculcural wast product. Endoglucanase enzyme is necessary of cellulase complex. Enzyme immobilization with ionic binding method, using DEAE-sephadex A-50 as its support could improved the enzyme specific activity. The optimum pH of the immobilized endoglucanase (C") remaianed constant, but the optimum temperature was increased to 55o C. The stability of the immobilized enzyloae when operated continously indicated that the activity enzyme could be maintained for 85 hours. After operating for about 120 hours the immobtlized enzyme activity was still about 50%. The stability of the immobilized enzyme whwn operated repeatedly for 10 times still indicated a erlzyme activity of. 7 0o/o.

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