Aplication of Liquid Chromatography - Polarimetric Detection to the Determination of Cyclodextrins in Mengkudu (Noni) Juice

Zein, Rahmiana and Munaf, Edison and Takeuchi, Toyohide (2010) Aplication of Liquid Chromatography - Polarimetric Detection to the Determination of Cyclodextrins in Mengkudu (Noni) Juice. Jurnal Sains dan Teknologi Farmasi, 7 (2). pp. 76-79. ISSN 1410-0177

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Abstract

Cyclodextrins (CDs) were separated and detected by liquid chromatography. The detection was based on optically polarimetric detection. The elution order of CDs on Develosil ODs-UG-5 column was γ-, α-, monoG1- β, and β -CD. The calibration graps of CDs were linear up to concentration 1mM, with the concentration detection limit of α-CD, β – CD, γ-CD and mono-G1-β-CD (S/N= 3) are 39, 52, 18 and 33 µM, respectively. Corresponding to the mass detectionlimit of of α-CD, β – CD, γ-CD and mono-G1-β-CD are 0.55, 0.96, 0.26, and 0.47 nmol. The method has been applied to the determination of CDs present in Mengkudu (Noni) juice where the concentration of γ-CD found in Mengkudu (Noni) juice is 0.028 µM. Keywords : Cyclodextrin, liquid chromatography, optically refactometry detector and Mengkudu juice.

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