TEKNIK FERMENTASI SUAPAN KELOMPOK (Fed-Batch) PADA BIOSINTESIS POLI (3-HIDROKSIBUTIRAT) DARI BAHAN DASAR MINYAK KELAPA SAWIT

Djamaan, Akmal and Madjid, Mohammed Isa Abdul and Noor, Azizan Mohamad (2010) TEKNIK FERMENTASI SUAPAN KELOMPOK (Fed-Batch) PADA BIOSINTESIS POLI (3-HIDROKSIBUTIRAT) DARI BAHAN DASAR MINYAK KELAPA SAWIT. Jurnal Sains dan Teknologi Farmasi, 7 (2). ISSN 1410 – 0177

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Abstract

A fed-batch fermentation technique on biosynthesis of a biodegradable plastic poly(3-hydroxybutyrate). P(3HB) using a mineral-media with palm oil as a single carbon source by Erwinia sp. USMI-20 has been carried out. Fermentation was conducted on a rotary shaker incubator under aerobic condition at pH 7.0, incubation temperature of 30ºC, and agitation rate of 200 rpm for 66 hours. A single feeding of 4.6 g/l palm oil was used at 30 hour cultivation. The characterization of the polymer production was based on cell growth, polymer content and polymer concentration. From this study, the maximum level of producing P(3HB) was at 66 hour cultivation with polymer content of 63 % b/b, polymer concentration of 3.53 g/l, and the dried cell weight of 5.6 g/l. Keywords : Fed-batch, poly(3-hydroxybutyrate), biodegradable plastic, palm oil, Erwinia sp. USMI-20

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