Application of Liquid Chromatography – Polarimetric Detection to The Determination of Cyclodextrins in Mengkudu (Noni) Juice

Zein , Rahmiana and Munaf, Edison and Takeuchi, Toyohide (2010) Application of Liquid Chromatography – Polarimetric Detection to The Determination of Cyclodextrins in Mengkudu (Noni) Juice. Jurnal Sains dan Teknologi Farmasi, 7 (2). ISSN 1410 – 0177

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Abstract

Cyclodextrins (CDs) were separated and detected by liquid chromatography. The detection was based on optically polarimetric detection. The elution order of CDs on Develosil ODS-UG-5 column was γ-, α-, monoG1-β-, and β-CD. The calibration graps of CDs were linear up to concentration 1 mM, with the concentration detection limit of α-CD, β-CD, γ-CD and monoG1-β-CD (S/N = 3) are 39, 52, 18 and 33 µM, respectively. Corresponding to the mass detection limit of α-CD, β-CD, γ-CD and monoG1-β-CD are 0.55, 0.96, 0.26, and 0.47 nmol. The method has been applied to the determination off CDs present in Mengkudu (Noni) juice where the concentration of γ-CD found in Mengkudu (Noni) juice is 0.028 µM. Keywords : Cyclodextrin, liquid chromatography, optically refractometry detector and mengkudu juice

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