Pengaruh Diabetes terhadap Haemodinamik dan Aktifitas Saraf Simpatik Ginjal pada Tikus Sprague-Dawley

Helmi, A. and Armenia, Armenia and Johns, E.J. and Munavvar, A.S and Yusof, APM (2014) Pengaruh Diabetes terhadap Haemodinamik dan Aktifitas Saraf Simpatik Ginjal pada Tikus Sprague-Dawley. Jurnal Sains dan Teknologi Farmasi, 8 (1). ISSN 1410 – 0177

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Abstract

This study sets up to examine the effect of diabetes on the haemodinamic and renal symphatic nerve activity in normal male Sprague-Dawley (SD) rats. Diabetes was chemically induced using streptozotocin (STZ) 60 mg/kg intraperitoneally, and the experiment was carried out seven days later. Body weight, total water intake, total urine output, urine sodium excretion were determined every 24 hours after STZ. The blood glucose, systolic and diastolic blood pressure (SBP and DBP), heart rate (HR) and renal sympathetic nerve activity (RSNA) were measured the day of acut experiment conducted. The animals were anaesthetized by a mixture of fluothane/O2 with flow rate 4 and 5 l/min for 2 min and there after maintained on chloralose/urethane (12 mg and 180 mg/ml) 0.1 ml/5 min(6-7 times) followed by 0.05 ml/30 min respectively, administered intravenously via femoral vein. Blood pressure (systolic and diastolic) and heart were monitored using a grass transducer connected to LabVIEW data acquisition system on a Macintosh computer. Renal nerves were isolated and placed on bipolar electrodes and their activity measured the above system. Blood pressure, heart rate and renal sympathetic nerve activity were measured. The same experiment was also done in the normal SD rats. Result showed that urine out flow, water intack, natrium urine and blood glucose levels were increased in diabetic state while body weight was redused. Blood pressure were increase, but HR was decrease. However on RSNA was increased in diabetic state. Collectively, these data indicated that diabetes can influence blood pressure, heart rate, renal sympathetic nerve activity in experimental diabetes rats. Keywords : diabetes, haemodinamik, saraf simpatik ginjal.

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