VARIASI GENETIK DENGAN TEKNIK RAPD PADA MUTAN PISANG KEPOK YAING TAHAN TERHADAP PENYAKIT BDB HASIL MUTASI INDUKSI DENGAN EMS

Mansyurdin, Mansyurdin and YantI, Yulmira (2010) VARIASI GENETIK DENGAN TEKNIK RAPD PADA MUTAN PISANG KEPOK YAING TAHAN TERHADAP PENYAKIT BDB HASIL MUTASI INDUKSI DENGAN EMS. In: Seminar dan Rapat Tahunan BKS-PTN Wilayah Barat ke-21, 10-11 May 2010, Universitas Riau.

	PDF (VARIASI GENETIK DENGAN TEKNIK RAPD PADA MUTAN PISANG KEPOK YAING TAHAN TERHADAP PENYAKIT BDB HASIL MUTASI INDUKSI DENGAN EMS) - Published Version Available under License Creative Commons Public Domain Dedication. Download (760Kb)
	Postscript (VARIASI GENETIK DENGAN TEKNIK RAPD PADA MUTAN PISANG KEPOK YAING TAHAN TERHADAP PENYAKIT BDB HASIL MUTASI INDUKSI DENGAN EMS) - Published Version Available under License Creative Commons Public Domain Dedication. Download (3247Kb)

Abstract

The mutant clone which resulted throught inducted mutation by EMS were seleted 5 resistant clones of banana cultivar Kepok againt to BDB. Analysis of genetic variation have done by RAPD technique and genetic change of mutant by DNA sequencing. The band profile is resulted DNA amplification of mutant clones and non-mutant of Kepok's cultivar by using 4 primers (0P801, 0PA02, OPA09 dan OPH07) were resulted 160 bands, but polymorphic bands are low relative (3,75%). Based on polymorphic band profile that indicates EMS treatment to cultivar Kepok were caused relative low genetic variation. The genetic change of mutant clone were showed by Query 301 dan Qaery 358, that is disappear several base of DNA sequence, that indicates the mutation caused a deletion of nucleotides. Keywords: cultivar Kepok of banana; EMS; mutant; RAPD, BDB

Actions (login required)

View Item