Pengaruh Perbedaan Rasio Karbon-Nitrogen pada Produksi P(3HB) Oleh Bakteri Bacillus brevis FAAC-202011

Djamaan , Akmal and Rustini, Rustini and Taufik , Imam (2010) Pengaruh Perbedaan Rasio Karbon-Nitrogen pada Produksi P(3HB) Oleh Bakteri Bacillus brevis FAAC-202011. Jurnal Sains dan Teknologi Farmasi, 8 (1). ISSN 1410 – 0177

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Abstract

The effect of the difference of mol ratio carbon-nitrogen (C/N) on production of a biodegradable plastic poly(3-hydroxybutirate), P(3HB) by Bacillus brevis FAAC-202011 has been studied. The C/N ratio were used of 5, 10, 15 and 20 by using glucose as carbon source and NH4Cl as nitrogen source. The fermentation process was conducted through the optimum condition of Bacillus brevis FAAC-202011 growth at pH 7, incubation temperature 30ºC, and agitation rate of 200 rpm for 60 hours. The characterization of the polymer production was based on cell growth, polymer content and plymer concentration. Result showed that optimum C/N ratio to produce P(3HB) was 15, with cultivation time was 56 hours, amount of dried cell was 104.4 mg, with polymer content, polymer concentration, and polymer percentage were 0.45 mg, 2.36 mg/100 ml and 2.26 weight % respectively. Keywords : C/N ratio, biodegradable plastic, poly(3-hydroxybutirate), glucose, and Bacillus brevis FAAC-202011

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