PERBANYAKAN GAMBIR (Uncariu gambir) MELALUI INDUKSI KALUS SECARA IN VITRO

Ferita, Istino and Satriao, Benni and Djafaruddin, Djafaruddin (2010) PERBANYAKAN GAMBIR (Uncariu gambir) MELALUI INDUKSI KALUS SECARA IN VITRO. Jurnal Stigma, 8 (1). pp. 12-16. ISSN 0853-3776

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Abstract

An experiment was carried out at the plant tissue culture Laboratory of Department of Agronomy, Faculty ttf Agri- culture, Andalas University, Padang from September to December 1999. The objective of the experiment was to obtain the best combination af 2,4-D and kinetin to in- crease callus induction, and combination of NAA, kinetin and BAP to increase callus generation of gambir in vifto. Two factor treatments lvere arranged in Completely Ran- domized Design (CRD) with 4 replications. The first factor was concentration of 2,4-D i.e:0,0; 0,5; and 1,0 ppm and the second factor was concentration of kinetin i.e :0,0; 0,5; 1,0; and 1,5 ppm. For stage callus regeneration, callus formed on stage callus induction were cultured on combi- nation concentration of 0,5 ppm NAA + 0,1 ppm kinetin + 3,0 ppm BAP. Observations were on percentage of browuing, percentage of callus, percentage of live, and percentage of shootlet. Result indicated that the interac- tion of concentration of 0,5 ppm, 2,4-D and 0,5 ppm ki- netin was the best in callus induction. For stage callus regeneration formed shootleto rootlet, and planlet on com- bination concentration of 0,5 ppm NAA + 0,1 ppm kinetin + 3,0 ppm BAP.

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