Efektivitas penggunaan kokultur sel-sel epitel tuba Fallopii dan kumulus dalam TCM-199 terhadap perkembangan embrio sapi in vitro)

Hendri, Hendri (2010) Efektivitas penggunaan kokultur sel-sel epitel tuba Fallopii dan kumulus dalam TCM-199 terhadap perkembangan embrio sapi in vitro). Project Report. Lembaga Penelitian Universitas Andalas. (Unpublished)

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Abstract

This research was designed to determine the effectivenerss of using cocultures of Fallopian tube epithelial and cumulus cells in Tissue Culture Medium 199 (TCM- 199) on in vitro bovine embryos development. Oocytes were collected by aspiration method from ovaries obtained from the slaughter house. Oocytes were classified into three grades (A, B and C/D) according to their qualities and only oocytes of A and B qualities (COCs, cumulus oocyte complexes) that matured in TCM-I99 supplemented with 20 percents of seven days after estrous heifer serum (SH7), at 38.5'C, 5.0% CO2 in humidifed air for 22 to 24 hours. Oocytes were then fertilized in vitro with capacitated spermatozoa from fresh semen. After six to eight hours of culturing, the presumptive zygotes or the fertilized oocytes were transferred and cultured into 0,1 ml drop of in vitro culture media treatment for development of embryos that called the control medium (TCM-199 + 20% SH7), comparing it to the ones cultured in coculture media of Fallopian tube epithelial and cumulus cells. These embryos were then incubated for 4 x 48 hours, evaluated and its culture medium was changed with a fresh one every 48 hours. Data from this experiment was transformed by vx 10,5 and analyzed by one way clasification test. Result from this experiment indicate that the coculture systems of Fallopian tube epithelial cells and of cumulus cells do not improve the morula rate.

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