Upaya Meningkatkan Keragaman Somaklonal Tanaman Penghasil Gaharu (Aquilaria malacensis Lamk) secara Kultur In Vitro

Putri, Nurwanita Ekasari (2008) Upaya Meningkatkan Keragaman Somaklonal Tanaman Penghasil Gaharu (Aquilaria malacensis Lamk) secara Kultur In Vitro. Project Report. Lembaga Penelitian Universitas Andalas. (Unpublished)

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Abstract

Experiment to investigate by Effort to improve somaclone variation plant Agarwood (Aquilaria mulaccensi L ) to in vitro was done in Mei to December 2008 at tissue cultur laboratory of Agriculture Faculty, University Andalas Padang and Institute Agriculture Bogor, Bogor. The experiment consist of two serries. The first serries about give concentration 2,4-D for induction callus, seven (7) level consist of : 0,00 ppm (a1 ); 5,00 ppm (Al); 10,00 ppm (A2);15,00 ppm (A3); 20,00 ppm (A4); 25,00 ppm (A5); dan 30,00 ppm (A6) The second serries about give combination concentration NAA and BAP for regeneration callus,seven (5) level consist of : 0,00 ppm NAA + 0,00 ppm BAP (K0); 5,00 ppm NAA + 5,00 ppm BAP(KI); 5,00 ppm NAA + 10,00 ppm BAP(K2); 5,00 ppm NAA +15,00 ppm BAP(K3) dan 5,00 ppm NAA + 20 ppm BAP (K4). The experiment used Completely Ramdomized Design (CRD) with six (replication). Concentration 10,00 ppm 2,4-D (A2) were supperior result than any another treatment the support growth to get explant life, callus formation. Combination concentration 5,00 ppm NAA + 5,00 ppm BAP (K1) were supperior result than any another treatment the support growth to get callus life, and regeneration callus formed shootlet. Key Words : Explant, Induction callus, Regeneration callus, Callus, 2,4-D, Auxin NAA, Cytokinin BAP, and in vitro.

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