PRODUKSt SENYAWA BIOpLASIK P(3HB) DARI MINYAK KELAPA SAWIT DALAM BIOREAKTOR KAPASITAS 100 LITER DAN PEMANFAATANNYA SEBAGAI KEMASAN RAMAH LINGKUNGAN

Djamaan, Akmal and Agustien , Anthoni (2014) PRODUKSt SENYAWA BIOpLASIK P(3HB) DARI MINYAK KELAPA SAWIT DALAM BIOREAKTOR KAPASITAS 100 LITER DAN PEMANFAATANNYA SEBAGAI KEMASAN RAMAH LINGKUNGAN. Project Report. LEMBAGA PENELITIAN UNAND. (Unpublished)

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Abstract

This research was initiated to study the ability of Erwima sp. USMI-26 to produce a bioplastic compound of P(3HB) from palm oil as a singte carbon sources by fermentation process. Fermentation was cunducted in a 100L bioreactor at pH 7, aeration 40 % v/v, agitation 200 rpm far 48 hours. The polymer granules accumulated in the cetls of Erwinia sp. USMI-20 upon cultivation were extracted and purified and characterized by using gas chromatography (GC), 1H and tuC nuclear magnetic resonance (NMR). The thermal properties including melting temperature (I,) and glass transition temperature (In) for the polymer were analyzed by differential scanning calorimetry (DSC). The molecular weight was determined by gel permeation chromatography (GPC). Our results showed that by using palm oil as the sole carbon source, Erwinia sp. USMI-2O was able to produce P(3HB) with a maximum potymer conten I of 45. 8 o/o of the dry cell weight with an optimum fermentation time of 48 hours. The G and In of polymer were of 175 oC and 15 oC respectively. The weight-average molecular weight (Mw) of the P(3HB) produced was 700.000-800.000 Da whereas the number-average (Mn) was 250.000-300.000 Da with a polydispersity index (Mw/Mn) of 2.3-2.8.

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