Deteksi Gen ctx pada Bakteri Vibrio cholerae Isolat Limbah Cair Rumah Sakit dan Uji Resistensinya Terhadap Beberapa Antibiotik

Marlina, Marlina and Almasdy, Dedy and Aufa, Insanil (2014) Deteksi Gen ctx pada Bakteri Vibrio cholerae Isolat Limbah Cair Rumah Sakit dan Uji Resistensinya Terhadap Beberapa Antibiotik. Jurnal Sains dan Teknologi Farmasi, 12 (2). ISSN 1410 – 0177

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Abstract

The characteristic of V. cholerae for ctx gene were observed by Polymerase Chain Reaction (PCR) method from several hospital waste water in Padang. Antibiotic resistances were tested for twenty cultures of Vibrio cholerae by Krumperman diffusion method for 13 kinds of antibiotics. Three of V. cholerae cultures were positives for ctx gene. The percentage of V.cholerae resistances to tetracycline, sulfamethoxazole, ampicillin, erythromycin, bacitracin, streptomycin, nalidixic acid, ceftriazone, nitrofurantoin, and gentamicin were of 95%, 85%, 75%, 70%, 50%, 25%, 25%, 20%, 15%, 10% respectively. There are no cultures were resistant to chloramphenicol, norfloxacin and kanamycin. Value of Multiple Antibiotic Resistant was of 0,362. Keywords : V. Cholerae, gen ctx, resistensi antibiotik

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