BIOAKTIFITAS DAN IDENTIFIKASI SENYAWA SIKLO DIPEPTIDA YANG DIHASILKAN OLEH Streptomyces sp A11

Sunaryanto, Rofiq and Marwoto, Bambang and Irawadi, Tun Tedja and Mas’ud , Zainal Alim and Hartoto, Liesbetini (2009) BIOAKTIFITAS DAN IDENTIFIKASI SENYAWA SIKLO DIPEPTIDA YANG DIHASILKAN OLEH Streptomyces sp A11. Working Paper. Farmasi. (Unpublished)

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Abstract

Determination of Minimum Inhibitory Concentration (MIC) and chemical structure elucidation of antibiotic produced by Streptomyces sp A11 have been carried out. Broth of antibiotic was obtained by liquid fermentation using Streptomyces sp A11. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Structure elucidation was carried out using ESI-MS, 1HNMR, 13CNMR, and 13C DEPT NMR. Pure antibiotic showed inhibition activity to gram negative and gram positive bacteria. MIC to Escherichia coli ATCC 25922 was 27 µg/mL, Pseudomonas aeroginosa ATCC27853 68,7 µg/mL, Staphylococcus aureus ATCC25923 80,2 µg/mL, and Bacillus Subtilis ATCC 66923 73,7 µg/mL. Identification using ESI-MS showed that molecular weight of this antibiotic was 260 g/mol, and molecule formula was C14H16N2O3. Elucidation of chemical structure using 1HNMR, 13CNMR, and 13C DEPT NMR showed that antibiotic was cyclo (tyrosyl-prolyl). They have maximum UV visible absorbance at 210 and 274.5 nm. Keywords : Antibiotic, Streptomyces, Minimum Inhibitory Concentration, Structure Elucidation

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