REGENERASI KALUS SAGU ( metroxylon so ) PADA BERBAGAI KOMBINASI KONSENTRASI AUKSIN DAN SITOKININ MELALUI TEKNIK KULTUR JARINGAN

Mayerni, Reni and Nugraha, Media Isma and Ferita, Istino (2010) REGENERASI KALUS SAGU ( metroxylon so ) PADA BERBAGAI KOMBINASI KONSENTRASI AUKSIN DAN SITOKININ MELALUI TEKNIK KULTUR JARINGAN. Working Paper. Fakultas Pertanian. (Unpublished)

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Abstract

An experiment on the callus regeneration of sago palm different combination of auxin and citocinin concentration by in –vitro was carried out at plant tissue culture lab. Of departement of Agronomy, Faculty of Agricultture, Andalas university, padang From Juni to Oktober 2001. The object of experiment was to obtain the best combination of NAA and BAP for Promoting callus generation of sago palm culture in- vitro. The experiments used the completely randomised design with eight packages of plant growth regulator i. E. A : 0,25 ppm NAA + 1,75 ppm BAP; B. 0,25 ppm NAA + 3,00 ppm BAP; C. 0,5 ppmNAA + 1,75 ppm BAP: D. 0,5 ppm NAA + 3,00 ppm BAP; E. 0,25 ppm NAA 1,75 ppm BAP + 0,1 ppm + 0,1 ppm cinnetin; F. 0,25 ppm NAA + 3,00 ppm BAP + 0,1 ppm cinetin; G. 0,5 ppm NAA + 1,75 ppm BAP + 0,1 ppm BAP cinetin; H. 0,5 ppm NAA 3,00 ppm BAP + 0,1 ppm cinetin. Result of this research indicate that auxin and citocinin gave no effecttoall of research observation parameter because no callaus induction tissue enlarged, developed and turned green on the media, but no callus induction or axilliary bud outgrowth has been obtained to date.

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