MICRODIALYSIS STUDY OF THE EFFECTS OF ASEBOTOXIN-III (ATX-III) ON THE METABOLISM OF NEUROTRANSMITTER OF BRAIN RATS

Oenzil, Fadil (2010) MICRODIALYSIS STUDY OF THE EFFECTS OF ASEBOTOXIN-III (ATX-III) ON THE METABOLISM OF NEUROTRANSMITTER OF BRAIN RATS. MICRODIALYSIS STUDY OF THE EFFECTS OF ASEBOTOXIN-III (ATX-III) ON THE METABOLISM OF NEUROTRANSMITTER OF BRAIN RATS, 23 (2). ISSN 0126 – 2092

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Abstract

The aims of this study is to investigate the alterations of neurotransmitters metabolisme and its metabolites after administration of ATX-III into striatum by using microdialysis technique. Adult male rats were subjects of in vivo brain microdialysis study. Brain striatum cannulated in anaesthetized animal experiment. After 20-24 hours the animals were perfused (2 ul/minute) with Ringer's solutions under free moving condition. The solutions were perfused first without and then with ATX-III (10 ug/ml Ringer's solution). The solution was perfused for at least 50 minutes to establish a steady state condition. Brain microdialysis and HPLC (ECD-100, EICOM Co., Kyoto) were perfomed with a fully automatic system. The HPLC Collums was MA-50DS (4.6x150 mm), the mobile phase was 0.1 M sodium citrate-acetate buffer (pH=3.5) containing 0.1 mM EDTA and 17% methanol. The effluent solution were collected every 25 minutes interval and were applied directly onto the HPLC-ECD apparatus. A typical data was showed after at least 50 minutes of steady state condition. The content of dopamine (DA) metabolites such as: DOPAC (3,4-dihydroxyphenyl acetic acid), HVA (homovanillic acid) and HMPG (4-hydroxy-3-methoxy phenylethylene glycol) decresed after injection of ATX-III into brain striatum. Serotonin (5HT=5-hudroxytriptamine) metabolites, 5 HIAA (5-hydrxyindoleacetic acid), also reduced markedly after ATX-III perfusion. But level of serotonim, DA and NE (Nor-epinephrin) in the effluent solution increased after ATX-III injection into brain striatum rat (n=3). High level of serotonin, DA and NE after direct injection of ATX-III into brain ventricle play an important role lung hemorrhage in experiment animal. Key word : asebotoxin, brain, microdialysis

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