Effects of Saponin in Sapindus rarak on Rumen Microbes

Ningrat, Rusmana WS (2014) Effects of Saponin in Sapindus rarak on Rumen Microbes. Working Paper. Fakultas Peternakan. (Unpublished)

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Abstract

The aim of the experiment was to examine the antiprotozoal activity of the pericarp from Sapindus rarak DC (Sapindaceae), well known for the high saponin content, for its potential to defaunate the rumen and hence enhancing rumen productivity. Raw material and extract of pericarp of S. rarak were screened for their effects on rumen protozoa and bacteria. The peri carp of S. rarak was extracted. The fractions were collected and monitored by TLC. The active compounds were tested in vitro for toxicity to rumen protozoa and on growth of pure cultures of rumen bacteria. The results showed that the saponin fractions of S. rarak exhibit antiprotozoal activity in vitro. Inclusion of methanol extract of S. rarak in the growth medium of pure cultures of rumen bacteria had no effect. In conclusion, S. rarak tested in this study has a great potential for suppressing rumen ciliate populations. Therefore, controlling rumen ciliate protozoa would be expected to lessen the dependence on protein supplementation which occurs frequently in animals receiving low-quality tropical forages. Keywords: Sapindus rarak, saponins, antiprotozoal activity, rumen bacteria.

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