Rusnam, Rusnam and Neni , Gusmanizar and Farrah Aini, Dahlan and Noor Azlina , Masdor
ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND AMIDE-DEGRADING BURKHOLDERIA SP. STRAIN NENI-11 IN SOILS FROM WEST SUMATERA, INDONESIA.
The IIOAB Journal-Institute of Integrative Omics and Applied Biotechnology, 7
(1).
pp. 28-40.
ISSN 0976-3104
Abstract
A molybdenum-reducing bacterium isolated from contaminated soil was able to utilize acrylamide as the
electron donor source, and was able utilize acrylamide, acetamide and propionamide for growth.
Reduction was optimal at pH between 6.0 to 6.3, at temperatures of between 30 and 37 oC, glucose as
the electron donor, phosphate at 5.0 mM, and sodium molybdate at 15 mM. The absorption spectrum of
the Mo-blue indicates it is a reduced phosphomolybdate. Molybdenum reduction was inhibited by
mercury (ii), silver (i) and chromium (vi) at 2 p.p.m. by 91.9, 82.7 and 17.4 %, respectively. Biochemical
analysis resulted in a tentative identification of the bacterium as Burkholderia cepacia strain Neni-11. The
growth of this bacterium modelled according to the modified Gompertz model. The growth parameters
obtained were maximum specific growth rates of 1.241 d-1, 0.971 d-1, 0.85 d-1 for acrylamide,
propionamide and acetamide, respectively, while the lag periods of 1.372 d, 1.562 and 1.639 d were
observed for acrylamide, propionamide and acetamide, respectively. The ability of this bacterium to
detoxify molybdenum and grown on toxic amides makes this bacterium an important tool for
bioremediation.
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