INDUKSI KALUS MANGGIS (Gareinia mangostane L.) MELALUI KULTUR IN VITRO(Inductinn Callas Mangosteen (Garcinia manga'stana f,.) By In Yitro Cultare)

Satria, Benni and Fauza, Hamda and Kaslil, Kaslil (2010) INDUKSI KALUS MANGGIS (Gareinia mangostane L.) MELALUI KULTUR IN VITRO(Inductinn Callas Mangosteen (Garcinia manga'stana f,.) By In Yitro Cultare). Stigma, VII (1). ISSN 0853-3776

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Abstract

Experiment induction callus mangosteen (Garcinia mangostana L.) by in vitro culture was done in August to December 1997 . The objective of this experiment was to get medium composition that exactly to support induction callus mangosteen by in vitro culture and to get plantlet. The experiment used Corn-pletely Randomized Design (CRD) with Five (5) replication. Stage induction callus on Woody Plant Medium (WPM) with combination concentration Auxin and Cy- tokinin plant growth regulator four (4) level treatment, consist of : l), 2.00 ppm 2.4-D + 2.00 ppm Ki-netin (Kr); 2), 1.00 ppm 2.4-D + 1.00 ppm NAA + 1.00 ppm Kinetin (Kj; 3), L.75 ppm Kinetin + 0,25ppm NAA (Kr); 4), 135 ppm Kinetin + 0.50 ppm NAA (K4). Combination Concentration 2.00 ppm 2.4-D + 2.00 ppm Kinetin (Kr) were superior result than any another treatment the support induction callus. Percentage explant browning and contamination were lower result on combination concentration 2.00 ppm 2.4-D + 2.00 ppm Kinetin(Kr) than any another freatment. Percentage explant life and callus formation were higher result on combination concentration 2.00 ppm 2.4-D + 2.00 ppm Kinetin (K1) than any another treatment. The result of this study show that effect medium composition WPM + 1,75 ppm BAP + 0,50 ppm NAA and effect medium composition WPM + 3,00 ppm BAP + 0,01 ppm Kinetin + 0,50 ppm NAA with plant organ cotil ring were supperior result than any other treatments on the inisition and proliferation of mangosteen in vitro culture.

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