DETERMINATION OF NITRATE AND NITRITE SPECTROPHOTOMETRICALLY BY NITRITE AZOTATION AND USING HYDRAZINE AS REDUCING AGENT

Suyani, Hanuar and Lestari, Intan and Alif , Admin and Munaf , Edison (2010) DETERMINATION OF NITRATE AND NITRITE SPECTROPHOTOMETRICALLY BY NITRITE AZOTATION AND USING HYDRAZINE AS REDUCING AGENT. Jurnal Kimia Andalas, 7 (1). ISSN 0853-8018

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Abstract

Nitrate and nitrite determination by spectrophotometry through nitrite azotation reaction was valuated. Nitrate was reduce into nitrit by hydrazine sulfate in basic solution using CuSO4 as catalyst. Nitrite was then reacted with sulfanilic acic and N-1-naphtyl amine in acid solution forming colored solution. The best reduction conditions were obtained at hydrazine concentration of 0,03 ml/L, CuSO4.5H2O concentration of 0,03 g/L and NaOH concentration of 0,5 mol/L, heated at 45ºC for 60 second. Azotation reaction was optimum at sulfanilic concentration of 6 %, naphthyl amine concentration of 0,3 %. Maximum absorbance was obtained at 520 nm and color was stabile for 3 hours. Relative standard deviations for six replications were 1,68 % for nitrate and 1,36 % for nitrite. Detection limits of the method were 0,015 mg/L for N-NO3 and 0,0067 mg/L for N-NO2. Recoveries obtained by spike samples were 101,2 % for nitrate and 105,7b % for nitrite.

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