Fermentasi Senyawa Bioaktif dari Jamur Trichoderma koningii untuk Mendapatkan Bahan Baku Fungisida Alamiah Baru

Djamaan, Akmal (2010) Fermentasi Senyawa Bioaktif dari Jamur Trichoderma koningii untuk Mendapatkan Bahan Baku Fungisida Alamiah Baru. Jurnal Sains dan Teknologi Farmasi, 7 (1). ISSN 1410 – 0177

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Abstract

The objective of this study is to produce bioactive component from Trichoderma koningii by fermentation process. The fermentation medium was used combination of corn water extract, sucrose, calcium carbonate, ferrosulphat, cobal chloride, and water up to 100 %. The fermentation was conducted at pH 7.0, agitation 180 rpm, temperature 20-25°C for 5 days. Activity of metabolite that produced was determinated toward Sclerotium rolfsii every 24 hours period during the fermentation process. Product of fermentation was extracted by liquid-liquid extraction method using chloroform, methyl acetate, n-buthanol, and water respectively. Each fraction with concentration 0.1; 0.5 and 1.0 % w/v were determined toward S. rolfsii. Result showed that the optimum time of fermentation occurred at 72 hours. The medium that produce highest activity is medium-A content of corn water extract 3 %, sucrose 3 %, calcium carbonate 0,5 %, ferrosulphate 0.1 % and water up to 100 %. The n-buthanol fraction refeal the highest activity toward S. rolfsii.

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