Isolasi Komponen Utama Fraksi Aktif “Brine Shrimp Test” dari Daun Anthocephalus cadamba Miq.

Rozani , Fauzia and Mukhtar , M. Husni and Bakhtiar , Amri and Arbain, Dayar (2010) Isolasi Komponen Utama Fraksi Aktif “Brine Shrimp Test” dari Daun Anthocephalus cadamba Miq. Jurnal Sains dan Teknologi Farmasi, 7 (1). ISSN 1410 – 0177

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Abstract

In continuation of our work on the chemistry of biologically active constituents of Sumatran plants it was found that the basic ethyl acetate fraction of the methanolic extract of the leaves of Anthocephalus cadamba Miq. showed significant activity with LC50 273,90 µg/ml when tested by “Brine Shrimp Lethality Assay”. This active fraction was then column chromatographed with step gradient polarity alution by using an increasing amount of ethyl acetate in hexane. Fractions having the same Rr values were combined, evaporated and tested for its activity. The active fraction was then rechromatographed, retested, purified and identified. From the active fraction was isolated as needles with a melting point of 268-269ºC and based on its spectroscopic data it was identified as ursolic acid and showed LC50 26,24 µg/ml, toward “Brine Shrimp Test”. The isomer of indole alkaloid compound was identified as valesiachotamine and isovalesiachotamine with LC50 > 1000 µg/ml. Keywords : Brine Shrimp Test, leaves of Anthocephalus cadamba Miq., LC50

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