OPTIMALISASI SISTEM PERBANYAKAN KACANG TANAH SECARA KULTUR JARINGAN PADA PROSES TRANSFER GEN (The optimization of in-vitro propagation of peanut in gene transfer process)

Anwar, Aswaldi (2010) OPTIMALISASI SISTEM PERBANYAKAN KACANG TANAH SECARA KULTUR JARINGAN PADA PROSES TRANSFER GEN (The optimization of in-vitro propagation of peanut in gene transfer process). Stigma, VIII (1). ISSN 0853-3776

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Abstract

The development of an effective gene transfer system for any crop depends largely on the ability to introduce a de- sired gene into the plant cells, the subsequent integration of the gene into the plant genome and on availabilify of tissue culture techniques that permit the growth and devel- opment of the explants and the recovery of fertile trans- genic plants. Gene transfer system in peanut is also de- pending on successfully of tissue culture techniques' espe- cially in the source of explants and the polarity of the ex- plants. The sources of explants in this experiment are the primary leaf of germinated embryo and the epicotyls. The treatment are; Primary leaves; horizontally epicofyls; ver- tically inverted epicotyls; and vertically upright epicotyls. Completcly Randomized Design was used as an experimental design with {ive observations. The disarmed super virulent Agrobacteritrn strain EHA 101 harboring pB1Hm IG was used for transformation studies. Result indicated that the source and polarity of the explants were critical as explants vertically inverted during co cultivation im- proved transformation and shoot regeneration liequency.

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