PENENTUAN ASAM ASKORBAT SECARA SPEKTROFOTOMETRI MENGGUNAKAN PENGOMPLEK O-FENANTROLIN (Determination of ascorbic acid spectrophotomet-rically using o-phenanthroline as a compexing agent)

Yusuf , Yulizar and Loekman , Umiati and Farit, Efrizon (2010) PENENTUAN ASAM ASKORBAT SECARA SPEKTROFOTOMETRI MENGGUNAKAN PENGOMPLEK O-FENANTROLIN (Determination of ascorbic acid spectrophotomet-rically using o-phenanthroline as a compexing agent). JUMPA JURNAL MATEMATIKA DAN PENGETAHUAN ALAM, 6 (1). ISSN 0853-0033

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Abstract

Determination of ascorbic acid with o-phenanthroline have been spectrophotometrically . Optimum condition was found at λ=508 nm, pH 5.0 - 6.0 reaction completion, after 10 minutes. The method have been used to determine the concentration of ascorbic acid in citrus fruits from Malee (guana juice). The result showed that fruit contained 227.6 ppm ascorbic acid with recovery of 100.96%, and relative standard deviation of 1.35%.

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