Studi Awal Pemisahan Amilosa dan Amilopektin Pati Singkong Dengan Fraksinasi Butanol – Air

Ben, Elfi Sahlan and Zulianis, Zulianis and Halim, Auzal (2014) Studi Awal Pemisahan Amilosa dan Amilopektin Pati Singkong Dengan Fraksinasi Butanol – Air. Jurnal Sains dan Teknologi Farmasi, 12 (1). ISSN 1410 – 0177

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Abstract

A Pleminary study on separation of amylose and amylopectin from cassava starch using fractination method with butanol – water (1 : 7) mixture has been done. Amylose 3.3 gram (11%) (hydro fraction) and amylopectin 4.25 gram (14%) (butanol fraction) were obtained from 30 gram cassava starch. Each fraction was evaluated for their physicochemical properties such as velocity of gelatination, iodine reaction, infrared spectra, water absorption and swelling time. Cassava starch, acid starch and soluble starch were used as refferenceS. The evaluation showed that the amylose fraction (hydro fraction) had the lowest velocity of gelatination and swelling, high water adsorption in comporison to amylopectin fraction (butanol fraction) and others. The iodine test of the amylose fraction (hydro fraction) showed a blue color, red color and for amylopectin fraction (butanol fraction), cassava starch, acid starch and soluble starch showed. Keywords : amylose, amylopectin, pati singkong.

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