Konfirmasi Gen tdh dan trh Secara PCR pada Bakteri V. parahaemolyticus dari Sampel Telur Chelonia mydas (penyu hijau) dan Eretmochelys imbricata (penyu sisik)

Marlina, Marlina and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Suharti, Netty and Desmawati, Desmawati (2009) Konfirmasi Gen tdh dan trh Secara PCR pada Bakteri V. parahaemolyticus dari Sampel Telur Chelonia mydas (penyu hijau) dan Eretmochelys imbricata (penyu sisik). Jurnal Sains dan Teknologi Farmasi, 14 (2). ISSN 1410 – 0177

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Abstract

Detection of toxR, tdh and trh genes in Vibrio parahaemolyticus from turtle eggs have been conducted. The confirmation test were done by using PCR methods. The presences of tdh and trh genes were considered as virulence factor. The results showed that turtle eggs from Chelonia mydas and Eretmochelys imbricata contained toxR gene 18.6 %, where eighteen of them contained tdh gene 8.4 % and none of them contained trh gene. KeyWord : Vibrio parahaemolyticus, gen tdh, gen trh, PCR

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